Cryo-Electron Microscopy Facility (Huck)

RRID:SCR_024456

Overview of Services

This Facility is built around the capabilities of our Thermo Fisher Titan Krios and Talos Arctica microscopes. Installed between Spring 2017 and Fall 2019, this revolutionary, cryo-based technology permits a full range of semi-automated applications, including 2D electron crystallography, single particle analysis, cryo-electron microscopy, and cellular tomography of frozen hydrated cell organelles and cells.

In 2025, the Hyra FIB-SEM is installed. This equipment has 4 ion beams that can provide faster lamella milling under cryo conditions. The addition of the IFLM fluorescence microscope enables the accurate targeting the cellular structures for lamella milling and slice viewing.

Leadership

Steering Committee

Wen Jiang, Faculty Director | Professor of Biochemistry and Molecular Biology
Katsu Murakami, | Professor of Biochemistry and Molecular Biology
Song Tan | Professor of Biochemistry and Molecular Biology
Clive Randall | Professor of Materials Science & Engineering, Director of the Materials Research Institute
Enrique Gomez | Professor of Chemical Engineering
Jean Paul Armache | Assistant Professor of Biochemistry and Molecular Biology

Location and hours of operation

Hours	Location
Monday - Friday 9:00 am - 5:00 pm 24/7 card swipe access for trained users	N-030-H, N-032 & N-034-A Millennium Science Complex University Park, PA 16802

Links and Resources

Cryo-EM Facility website
CORES (CrossLab) support, hosted by ORIS
EMDataBank Unified Data Resource for 3-Dimensional Electron Microscopy
EMPIAR Electron Microscopy Public Image Archive

Contacts

Name	Role	Phone	Email	Location
Sung Hyun (Joseph) Cho	Co-director, CryoEM Facility	814-865-5729	sxc73@psu.edu	W249 Millennium Science Complex
Jenny Wang	Co-director, Cryo-EM Facility	8148651158	zw691551@psu.edu	W252 Millennium Science Complex

Service list

Name	Description	Price
Amira	Per hour	Inquire
Consultation	Per hour	Inquire
Cu 300 mesh grid with Formvar - Carbon Type A (Ted Pella #01821)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid - No Formvar - Open Holes (Ted Pella #01895-F)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid with ultra-thin carbon film (Ted Pella #01824)	Per each	Internal $4.24 each
Grid storage box with pin type lid - holds 4 vitrified girds (Ted Pella # 160-42)	Per each	Inquire
Quantifoil Grid 1.2/1.3 on Cu 300 mesh (Ted Pella #658-300-CU )	Per each	Internal $6.51 each
Quantifoil Grid 2/1 on Cu 300 mesh (Ted Pella #661-300-CU-100)	Per each	Internal $7.11 each
Quantifoil Grid 2/2 on Cu 300 mesh (Ted Pella #657-300-CU-100)	Per each	Internal $6.51 each
Training - Computational Biology	Per hour	Inquire
Training - Microscopy and Sample Preparation	Per hour	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Consumables (7)
Cu 300 mesh grid with Formvar - Carbon Type A (Ted Pella #01821)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid - No Formvar - Open Holes (Ted Pella #01895-F)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid with ultra-thin carbon film (Ted Pella #01824)	Per each	Internal $4.24 each
Grid storage box with pin type lid - holds 4 vitrified girds (Ted Pella # 160-42)	Per each	Inquire
Quantifoil Grid 1.2/1.3 on Cu 300 mesh (Ted Pella #658-300-CU )	Per each	Internal $6.51 each
Quantifoil Grid 2/1 on Cu 300 mesh (Ted Pella #661-300-CU-100)	Per each	Internal $7.11 each
Quantifoil Grid 2/2 on Cu 300 mesh (Ted Pella #657-300-CU-100)	Per each	Internal $6.51 each
▼ ► Microscopy Services (4)
Name	Description	Price
Amira	Per hour	Inquire
Training - Computational Biology	Per hour	Inquire
Training - Microscopy and Sample Preparation	Per hour	Inquire
Consultation	Per hour	Inquire