Cryo-Electron Microscopy Facility (Huck)

RRID:SCR_024456

Overview of Services

This Facility is built around the capabilities of our Thermo Fisher Titan Krios and Talos Arctica microscopes. Installed betwwen Spring 2017 and Fall 2019, this revolutionary, cryo-based technology permits a full range of semi-automated applications, includng 2D electron crystallography, single particle analysis, cryo-electron microscopy, and dual axis cellular tomography of frozen hydrated cell organelles and cells.

EXTERNAL USERS: An Academic Research Services Order must be in place before we can accept your projects.

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Leadership

Steering Committee

Katsu Murakami, Faculty Director | Professor of Biochemistry and Molecular Biology
Song Tan | Professor of Biochemistry and Molecular Biology
Clive Randall | Professor of Materials Science & Engineering, Director of the Materials Research Institute
Enrique Gomez | Professor of Chemical Engineering
Jean Paul Armache | Assistant Professor of Biochemistry and Molecular Biology
Nigel Deighton | Director of Research Instrumentation, Huck Institutes of the Life Sciences

Location and hours of operation

Hours	Location
Monday - Friday 9:00 am - 5:00 pm 24/7 card swipe access for trained users	N-030-H, N-032 & N-034-A Millennium Science Complex University Park, PA 16802

Links and Resources

Cryo-EM Facility website
CORES (CrossLab) support, hosted by ORIS
EMDataBank Unified Data Resource for 3-Dimensional Electron Microscopy
EMPIAR Electron Microscopy Public Image Archive

Contacts

Name	Role	Phone	Email	Location
Sung Hyun (Joseph) Cho	Director, CryoEM Facility	814-863-2773	sxc73@psu.edu	W231 Millennium Science Complex
Ibrahim Moustafa		814-865-573	ria2@psu.edu

Service list

Name	Description	Price
Consultation	Per hour	Inquire
Cu 300 mesh grid with Formvar - Carbon Type A (Ted Pella #01821)	Per each	Internal $2.26 each
Cu 300 mesh Lacey Carbon grid - No Formvar - Open Holes (Ted Pella #01895-F)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid with ultra-thin carbon film (Ted Pella #01824)	Per each	Internal $4.24 each
Grid storage box with pin type lid - holds 4 vitrified girds (Ted Pella # 160-42)	Per each	Inquire
Model building	Computational model building from cryo EM data. Charged on the basis of hands-on labor, typically 24 hours, but applied for actual effort.	Inquire
Quantifoil Grid 1.2/1.3 on Cu 300 mesh (Ted Pella #658-300-CU )	Per each	Internal $6.51 each
Quantifoil Grid 2/1 on Cu 300 mesh (Ted Pella #661-300-CU-100)	Per each	Internal $7.11 each
Quantifoil Grid 2/2 on Cu 300 mesh (Ted Pella #657-300-CU-100)	Per each	Internal $6.51 each
Training - Computational Biology	Per hour	Inquire
Training - Microscopy and Sample Preparation	Per hour	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Consumables (7)
Cu 300 mesh grid with Formvar - Carbon Type A (Ted Pella #01821)	Per each	Internal $2.26 each
Cu 300 mesh Lacey Carbon grid - No Formvar - Open Holes (Ted Pella #01895-F)	Per each	Inquire
Cu 300 mesh Lacey Carbon grid with ultra-thin carbon film (Ted Pella #01824)	Per each	Internal $4.24 each
Grid storage box with pin type lid - holds 4 vitrified girds (Ted Pella # 160-42)	Per each	Inquire
Quantifoil Grid 1.2/1.3 on Cu 300 mesh (Ted Pella #658-300-CU )	Per each	Internal $6.51 each
Quantifoil Grid 2/1 on Cu 300 mesh (Ted Pella #661-300-CU-100)	Per each	Internal $7.11 each
Quantifoil Grid 2/2 on Cu 300 mesh (Ted Pella #657-300-CU-100)	Per each	Internal $6.51 each
▼ ► Microscopy Services (4)
Name	Description	Price
Model building	Computational model building from cryo EM data. Charged on the basis of hands-on labor, typically 24 hours, but applied for actual effort.	Inquire
Training - Computational Biology	Per hour	Inquire
Training - Microscopy and Sample Preparation	Per hour	Inquire
Consultation	Per hour	Inquire