Genome Sciences (COM)

Overview of Services

The full-service Penn State College of Medicine Genome Sciences Facility provides consultation, instrumentation and services to both Penn State and non-Penn State investigators in genomic, epigenomic and transcriptomic studies.

The variety of instrumentation allows for capabilities ranging from highly focused analysis of candidate SNPs and mRNAs to whole genome, exome, epigenome and transcriptome sequencing. Services are also available for a variety of study designs extending from a few laboratory samples to large clinical projects involving hundreds or thousands of samples. The full bioinformatics service is also available for data analysis.

We receive either tissue, DNA/RNA, or customer-generated NGS libraries. We process samples according based on agreement reached during consultations on the design of the experiment. We develop new applications to accommodate state-of-the-art NGS technologies. We conduct sequence read alignment, secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up with the interpretation of the results. We provide support for grant writing and hands-on training for students and postdocs in NGS processing.

Leadership

Sirisha Pochareddy, Ph.D. | Director

Location and hours of operation

Hours

Location

Monday - Friday

8:00 am - 4:30 pm

Room C2705

500 University Dr.

Hershey, PA, 17033

Links and Resources

Contacts

Name	Role	Phone	Email	Location
General Inquiry		717-531-5823	GenomeSciences@pennstatehealth.psu.edu	C2705

Service list

Name	Description	Price
10X Genomics 3' single cell RNA-seq (per sample, without kit)	Only library prep service DOES NOT include kit or sequencing costs; Kit will be added as a pass through charge Please contact the core for sample requirements	Inquire
NovaSeq SP (200 cycles )	750M reads per flow cell (though Illumina does not provide this kit we can accommodate customers that want to run an SP 200), the price will be set as the average between SP 100 and SP 300.	Inquire
shRNA	The Genome Sciences core offers access to our shRNA libraries to investigators upon request. Approximately 90,000 shRNA clones that target greater than 15,000 individual genes are available for either mouse or human genomes. shRNA is stored in -80º. Investigators will need to provide a summary of the specific clone they are requesting and will need to provide the LB broth. Core staff will pipette a small amount of the appropriate shRNA clone into the broth for the customer.	Inquire
Whole Genome Library prep, PCR free	Libray prep. only Sequencing not included Recomended sequencing depth 30X, 2x150 reads.	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► LibPrep Services (2)
10X Genomics 3' single cell RNA-seq (per sample, without kit)	Only library prep service DOES NOT include kit or sequencing costs; Kit will be added as a pass through charge Please contact the core for sample requirements	Inquire
Whole Genome Library prep, PCR free	Libray prep. only Sequencing not included Recomended sequencing depth 30X, 2x150 reads.	Inquire
▼ ► NovaSeq Services (1)
Name	Description	Price
NovaSeq SP (200 cycles )	750M reads per flow cell (though Illumina does not provide this kit we can accommodate customers that want to run an SP 200), the price will be set as the average between SP 100 and SP 300.	Inquire
▼ ► Other Services (1)
Name	Description	Price
shRNA	The Genome Sciences core offers access to our shRNA libraries to investigators upon request. Approximately 90,000 shRNA clones that target greater than 15,000 individual genes are available for either mouse or human genomes. shRNA is stored in -80º. Investigators will need to provide a summary of the specific clone they are requesting and will need to provide the LB broth. Core staff will pipette a small amount of the appropriate shRNA clone into the broth for the customer.	Inquire