The full-service Penn State College of Medicine Genome Sciences Facility provides consultation, instrumentation and services to both Penn State and non-Penn State investigators in genomic, epigenomic and transcriptomic studies.
The variety of instrumentation allows for capabilities ranging from highly focused analysis of candidate SNPs and mRNAs to whole genome, exome, epigenome and transcriptome sequencing. Services are also available for a variety of study designs extending from a few laboratory samples to large clinical projects involving hundreds or thousands of samples. The full bioinformatics service is also available for data analysis.
We receive either tissue, DNA/RNA, or customer-generated NGS libraries. We process samples according based on agreement reached during consultations on the design of the experiment. We develop new applications to accommodate state-of-the-art NGS technologies. We conduct sequence read alignment, secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up with the interpretation of the results. We provide support for grant writing and hands-on training for students and postdocs in NGS processing.
10x Genomics 3' single cell RNA-seq (Sample 2-8, without kit)
Only library prep service;
DOES NOT include kit or sequencing costs; Kit will be added as a pass through charge
Please contact the core for sample requirements
Inquire
10x Genomics 3' single cell RNA-seq (1st Sample, without kit)
Only library prep service;
DOES NOT include kit or sequencing costs; Kit will be added as a pass through charge
Please contact the core for sample requirements
Inquire
10x Genomics Library Prep (1st sample)
Single Cell RNA-Seq 3P Library Prep (V3, per sample, sequencing not included)
Inquire
10x Genomics Library Prep (Sample 2-8)
Single Cell RNA-Seq 5P Library Prep (per sample, sequencing not included)
Single Cell ATAC-Seq Library Prep (per sample, sequencing not included)
Single Cell RNA-Seq 3P Library Prep (V3, per sample, sequencing not included)
Inquire
Kapa Library prep for 16S rRNA: for 16S libary prep
Libray prep. only (Please note sequencing is not included in the library prep price).
we only accept minimum of 24 samples.
Inquire
NovaSeq_SP ( 200 cycles - SE 1x200, PE 2x100 ) (per flow cell)
750M reads per flow cell (though Illumina does not provide this kit we can accommodate customers that want to run an SP 200), the price will be set as the average between SP 100 and SP 300.
Inquire
shRNA
The Genome Sciences core offers access to our shRNA libraries to investigators upon request. Approximately 90,000 shRNA clones that target greater than 15,000 individual genes are available for either mouse or human genomes.
shRNA is stored in -80º. Investigators will need to provide a summary of the specific clone they are requesting and will need to provide the LB broth. Core staff will pipette a small amount of the appropriate shRNA clone into the broth for the customer.
Inquire
small (micro) RNA Library prep (NextFlex or Qiagen Kit) recomended mimimum 5-10M reads.
Library Prep only, sequencing not included
Inquire
Whole Genome Library prep (Nextera Flex)
Libray prep. only (Please note sequencing is not included in the library prep price, sequencing will be charged as fraction of the flow cell).
NovaSeq_SP ( 200 cycles - SE 1x200, PE 2x100 ) (per flow cell)
750M reads per flow cell (though Illumina does not provide this kit we can accommodate customers that want to run an SP 200), the price will be set as the average between SP 100 and SP 300.
The Genome Sciences core offers access to our shRNA libraries to investigators upon request. Approximately 90,000 shRNA clones that target greater than 15,000 individual genes are available for either mouse or human genomes.
shRNA is stored in -80º. Investigators will need to provide a summary of the specific clone they are requesting and will need to provide the LB broth. Core staff will pipette a small amount of the appropriate shRNA clone into the broth for the customer.
Welcome!
