Institute for Personalized Medicine (COM)

Measure dsDNA concentration using fluorometric reagent and assay tube.

Enter volume of 10 mM dNTP used in PCR Anchored Library prep reaction in ul.

Enter volume of 100% Ethanol used for sparQ SPRI washes in ml.

Ethyl Alcohol, 200 proof

Price per ml

Reagent cost per DNA sample.

AmpliSeq library preparation for illumina using the BRCA Panel for somatic and germline mutation detection.

Amplification based target enrichment.

Reagent cost per DNA sample.

AmpliSeq library preparation for Illumina using the Cancer Hotspot Panel v2.

Amplification based target enrichment.

Select this item to reimburse the IPM for the cost of reagents.

Includes Bionano Prep SP BMA, Blood and Cell, or Tissue DNAIsolation Kits. 

Thermo Fisher Scientific's Countess cell counting chambers.

Two chambers available per slide.

Select this item to reimburse the IPM for the cost of reagents.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Zymo Research DNA Clean & Concentrator-25.

Utilize for DNA concentration and/or buffer change required for downstream enzymatic processes.

Allows for RNase A treatment of DNA samples, using column based purification to remove enzyme.

For DNAs between 50 bp-23 Kb.

For purification of total DNA from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects

NanoEnTek's Eve cell counting slides.

Two chambers available per slide.

Zymo Research genomic DNA Clean & Concentrator-25

Utilize for gDNA concentration and/or buffer change required for downstream enzymatic processes.

Allos for RNase A treatment of DNA samples, using column based purification to remove enzyme.

For DNAs between 50 bp-200 Kb.

For White Blood Cell counts.

Charge necessary for AmpliSeq for Illumina BRCA Library batching, per batch of ≤48 DNAs.

AmpliSeq library preparation for Illumina using the BRCA Panel for somatic and germline mutations in BRCA1 and BRCA2.

Amplification based target enrichment.

Charge necessary for AmpliSeq Cancer HotSpot Panel v2 batching, per batch of ≤96 DNAs.

AmpliSeq library preparation for Illumina using the Cancer Hotspot Panel v2.

Amplification based target enrichment.

Enter volume of Phusion Hot Start II enzyme used in PCR Anchored Library prep reaction in ul.

Phusion II HIgh Fidelity HotStart Thermo Fisher Scientific F549S

50 units/50 ul 

Invitrogen QuantiT PicoGreen dsDNA Assay Kit, Quantity: 1mL kit, Assay: dsDNA Quantitation, Quantitation
Range: 50 pg2 ug, Detection Method: Fluorescent, Format: Kit, For Use With: Microplate Reader, No. of
Reactions: 200 assays (2 mL assay volume)

Price per ml is listed.

Cost of 384-well qPCR plate.

Necessary for qPCR runs.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Placeholder until I get more information on the kit and the additives I need to include in the pricing.

Cell-free and nuclear DNA isolation of ≥100 bp fragments.

Validated sample types include serum, plasma, amniotic fluid, and saliva.

Price per DNA sample and DNA dilution.

RNase P TaqMan Detection Kit quantifies a single copy gene by comparison to human DNA standards.

Cost is listed for the price per well. Total wells used is calculated by the number of standards, DNA samples and dilutions tested, and replicate values. Recommended testing for amplifiable DNA is performed in triplicate for 7 human DNA standards, 1 NTC, and 2 DNA dilutions.

RNase P DNA Quantification is performned in Batch:

Cost of PCR plate and film (IPM)

Cost of RNase P Detection Reagent (IPM)

Cost of TaqMan Expression Master Mix (GS)

Cost to run a plate in Genome Sciences (GS)

Select this item to reimburse the IPM for the cost of reagents.

Three samples must be processed on one Saphyr chip as there are 3 flow cells within.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Enter volume of sparQ SPRI used in ul.

QuantaBio 95196-060 60ml/VWR 76302-832

volume of sparQ SPRI used.

Price per well; for a DNA sample, dilution or replicate.

Quantitect Sybr Green, 2x; 384 well plate, 10 ul reactions.

DNA oligos, plate and film, QuantStudio 12K flex, are not included. 

Dr. Goldenberg prepurchased this item and it is not for use by anyone other than his research fellows.  Thank you.

Enter volume of nuclease free water used for sparQ SPRI washes here in ml.

Placeholder until I get more information on the kit and the additives I need to include in the pricing.

Zymo Research DNA Clean & Concentrator-25.

Utilize for DNA concentration and/or buffer change required for downstream enzymatic processes.

Allows for RNase A treatment of DNA samples, using column based purification to remove enzyme.

For DNAs between 50 bp-23 Kb.

For purification of total DNA from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects

Zymo Research genomic DNA Clean & Concentrator-25

Utilize for gDNA concentration and/or buffer change required for downstream enzymatic processes.

Allos for RNase A treatment of DNA samples, using column based purification to remove enzyme.

For DNAs between 50 bp-200 Kb.

Cell-free and nuclear DNA isolation of ≥100 bp fragments.

Validated sample types include serum, plasma, amniotic fluid, and saliva.

Invitrogen QuantiT PicoGreen dsDNA Assay Kit, Quantity: 1mL kit, Assay: dsDNA Quantitation, Quantitation
Range: 50 pg2 ug, Detection Method: Fluorescent, Format: Kit, For Use With: Microplate Reader, No. of
Reactions: 200 assays (2 mL assay volume)

Price per ml is listed.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Cost of 384-well qPCR plate.

Necessary for qPCR runs.

Price per well; for a DNA sample, dilution or replicate.

Quantitect Sybr Green, 2x; 384 well plate, 10 ul reactions.

DNA oligos, plate and film, QuantStudio 12K flex, are not included. 

Price per DNA sample and DNA dilution.

RNase P TaqMan Detection Kit quantifies a single copy gene by comparison to human DNA standards.

Cost is listed for the price per well. Total wells used is calculated by the number of standards, DNA samples and dilutions tested, and replicate values. Recommended testing for amplifiable DNA is performed in triplicate for 7 human DNA standards, 1 NTC, and 2 DNA dilutions.

RNase P DNA Quantification is performned in Batch:

Cost of PCR plate and film (IPM)

Cost of RNase P Detection Reagent (IPM)

Cost of TaqMan Expression Master Mix (GS)

Cost to run a plate in Genome Sciences (GS)

Reagent cost per DNA sample.

AmpliSeq library preparation for illumina using the BRCA Panel for somatic and germline mutation detection.

Amplification based target enrichment.

Charge necessary for AmpliSeq for Illumina BRCA Library batching, per batch of ≤48 DNAs.

AmpliSeq library preparation for Illumina using the BRCA Panel for somatic and germline mutations in BRCA1 and BRCA2.

Amplification based target enrichment.

Reagent cost per DNA sample.

AmpliSeq library preparation for Illumina using the Cancer Hotspot Panel v2.

Amplification based target enrichment.

Charge necessary for AmpliSeq Cancer HotSpot Panel v2 batching, per batch of ≤96 DNAs.

AmpliSeq library preparation for Illumina using the Cancer Hotspot Panel v2.

Amplification based target enrichment.

NanoEnTek's Eve cell counting slides.

Two chambers available per slide.

Thermo Fisher Scientific's Countess cell counting chambers.

Two chambers available per slide.

For White Blood Cell counts.

Select this item to reimburse the IPM for the cost of reagents.

Includes Bionano Prep SP BMA, Blood and Cell, or Tissue DNAIsolation Kits. 

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Dr. Goldenberg prepurchased this item and it is not for use by anyone other than his research fellows.  Thank you.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Select this item to reimburse the IPM for the cost of reagents.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Measure dsDNA concentration using fluorometric reagent and assay tube.

Select this item to reimburse the IPM for the cost of reagents.

Three samples must be processed on one Saphyr chip as there are 3 flow cells within.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

Enter volume of Phusion Hot Start II enzyme used in PCR Anchored Library prep reaction in ul.

Phusion II HIgh Fidelity HotStart Thermo Fisher Scientific F549S

50 units/50 ul 

Enter volume of 10 mM dNTP used in PCR Anchored Library prep reaction in ul.

Enter volume of sparQ SPRI used in ul.

QuantaBio 95196-060 60ml/VWR 76302-832

Enter volume of 100% Ethanol used for sparQ SPRI washes in ml.

Ethyl Alcohol, 200 proof

Price per ml

Enter volume of nuclease free water used for sparQ SPRI washes here in ml.

Please select this item for the ALL project in collaboration with Drs. Broach and Dovat.

Kitted reagent part of 30 genome bundle purchased for this specific project.

Bundle purchased on PO 802560.

volume of sparQ SPRI used.